van den Heuvel S, Harlow E
Massachusetts General Hospital Cancer Center, Charlestown 02129.
Science. 1993 Dec 24;262(5142):2050-4. doi: 10.1126/science.8266103.
The key cell-cycle regulator Cdc2 belongs to a family of cyclin-dependent kinases in higher eukaryotes. Dominant-negative mutations were used to address the requirement for kinases of this family in progression through the human cell cycle. A dominant-negative Cdc2 mutant arrested cells at the G2 to M phase transition, whereas mutants of the cyclin-dependent kinases Cdk2 and Cdk3 caused a G1 block. The mutant phenotypes were specifically rescued by the corresponding wild-type kinases. These data reveal that Cdk3, in addition to Cdc2 and Cdk2, executes a distinct and essential function in the mammalian cell cycle.
关键的细胞周期调节因子Cdc2属于高等真核生物中细胞周期蛋白依赖性激酶家族。利用显性负性突变来研究该家族激酶在人类细胞周期进程中的需求。显性负性Cdc2突变体使细胞在G2期到M期转换时停滞，而细胞周期蛋白依赖性激酶Cdk2和Cdk3的突变体则导致G1期阻滞。突变体表型可被相应的野生型激酶特异性挽救。这些数据表明，除了Cdc2和Cdk2之外，Cdk3在哺乳动物细胞周期中执行独特且必不可少的功能。
