Rosenblatt J, Gu Y, Morgan D O
Department of Physiology, University of California, San Francisco 94143-0444.
Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2824-8. doi: 10.1073/pnas.89.7.2824.
We have analyzed the cell cycle regulation of human cyclin-dependent kinase 2 (CDK2), a protein closely related to the cell cycle-regulatory protein kinase CDC2. We find that CDK2 activity, like that of CDC2, oscillates during the cell cycle in cultured mammalian fibroblasts. Unlike CDC2 activity (which peaks during mitosis), CDK2 activity rises in late G1 or early S phase and declines during mitosis. Active S-phase CDK2 migrates in multiple large complexes on gel filtration, and CDK2 in one of these complexes is associated with cyclin A. These findings suggest that CDK2 and CDC2, in association with distinct cyclins, regulate separate functions in the mammalian cell cycle.
我们分析了人类细胞周期蛋白依赖性激酶2（CDK2）的细胞周期调控，CDK2是一种与细胞周期调控蛋白激酶CDC2密切相关的蛋白质。我们发现，与CDC2一样，CDK2的活性在培养的哺乳动物成纤维细胞的细胞周期中振荡。与CDC2活性（在有丝分裂期间达到峰值）不同，CDK2活性在G1晚期或S期早期升高，并在有丝分裂期间下降。活跃的S期CDK2在凝胶过滤中以多个大复合物形式迁移，其中一个复合物中的CDK2与细胞周期蛋白A相关。这些发现表明，CDK2和CDC2与不同的细胞周期蛋白结合，在哺乳动物细胞周期中调节不同的功能。
