Speer C A, Marchiondo A A, Duszynski D W, File S K
J Parasitol. 1976 Dec;62(6):984-7.
Sporocysts of Isospora endocallimici, a parasite of marmosets, were exposed to minimal essentials medium (MEM) or a trypsin-bile salt solution (TBS) and then fixed and prepared for transmission electron microscopy. Excystation occurred in TBS but not MEM. The sporocyst wall has 2 layers, a thin outer layer (15 to 110 nm thick) and a thick inner layer (65 to 180 nm thick), which is composed of 4 separate curved plates. The outer layer consists of 1 to 3 membranes interspersed with lipid droplets. In the inner layer, a thin layer of material connects the peripheral margins of 2 apposing plates. Immediately beneath this layer, a thin strip of material is interposed between the 2 apposing plates. Ultrastructural changes preparatory to excystation occur primarily in the inner layer of the sporocyst wall. The TBS acts upon the site of apposition between 2 plates causing the interposed strip to swell and separate from the margin of each plate which leads to collapse of the sporocyst. As the sporocyst collapses, the margins of each curved plate curl inward toward the center of the sporocyst.
绒猴寄生虫内卡里米氏等孢球虫的孢子囊被置于最低限度基本培养基（MEM）或胰蛋白酶 - 胆盐溶液（TBS）中，然后固定并制备用于透射电子显微镜观察。脱囊在TBS中发生，但不在MEM中发生。孢子囊壁有两层，一层薄的外层（15至110纳米厚）和一层厚的内层（65至180纳米厚），内层由4个分开的弯曲板组成。外层由1至3层膜与脂滴相间构成。在内层，一层薄物质连接两个相对板的周边边缘。紧接这一层下方，一条薄带物质夹在两个相对板之间。脱囊前的超微结构变化主要发生在孢子囊壁的内层。TBS作用于两个板之间的贴合部位，使夹在中间的条带肿胀并与每个板的边缘分离，从而导致孢子囊塌陷。随着孢子囊塌陷，每个弯曲板的边缘向内卷曲朝向孢子囊的中心。
