Patkin E L, Kustova M E, Dyban A P
Tsitologiia. 1993;35(9):12-7.
The frequency of sister-chromatid exchanges (SCE) was analysed in spontaneously and superovulated morulae and blastocyst of CBA, C57Bl, F1 CBA x C57Bl mice in culture with bromodeoxyuridine (BrdU). The background level for SCE was found to be 4-5 times higher in early embryos than in fetal or adult tissues of the mouse. The phenomenon of spontaneous sister-chromatid differentiation (SCD) of blastocyst chromosomes without any treatment with BrdU was observed. The SCE frequency was calculated on such chromosomes and was the same as with BrdU. A possible mechanism of this phenomenon is suggested.
在含有溴脱氧尿苷（BrdU）的培养液中，分析了CBA、C57Bl、F1 CBA×C57Bl小鼠自发排卵及超排卵的桑椹胚和囊胚中姐妹染色单体交换（SCE）的频率。发现早期胚胎中SCE的背景水平比小鼠的胎儿或成体组织高4 - 5倍。观察到囊胚染色体在未用BrdU处理的情况下出现自发姐妹染色单体分化（SCD）现象。计算了这些染色体上的SCE频率，其与使用BrdU时相同。文中提出了这一现象的可能机制。
