Ultrastructural arrangement of collagen fibrils in the rat facial nerve.

We studied the collagen fibril arrangement in the connective tissue sheaths (epineurium, perineurium, and endoneurium) of the intratemporal and extra-temporal portion of the rat facial nerve by transmission electron microscopy after fixing the nerves with tannic acid, and by scanning electron microscopy after digesting cellular elements by sodium hydroxide treatment. These studies revealed that the epineurium consists of thick bundles of collagen fibrils and a coarse meshwork of collagen fibrils, the perineurium consists of a lacework sheet of bundles of collagen fibrils, and the endoneurium consists of a meshwork of fine collagen fibrils in the inner layer and of longitudinally oriented bundles of collagen fibrils in the outer layer. There was little difference in the three-dimensional organization of collagen fibrils between the extra-temporal segment and vertical and horizontal segments of the facial nerve. However, the facial nerve lost the epineurium and perineurium in the central portion of the horizontal segment near the geniculate ganglion and in the labyrinthine portion, i.e. the proximal portion of the intratemporal segment. In these segments, the endoneurium consisted of a meshwork of fine collagen fibrils.