Ushiki T, Ide C
Department of Anatomy, School of Medicine, Iwate Medical University, Morioka, Japan.
Cell Tissue Res. 1990 Apr;260(1):175-84. doi: 10.1007/BF00297503.
The organization of collagen fibrils in the rat sciatic nerve was studied by scanning electron microscopy after digestion of cellular elements by sodium hydroxide treatment, and by conventional transmission electron microscopy. The epineurium consisted mainly of thick bundles of collagen fibrils measuring about 10-20 microns in width; they were wavy and ran slightly obliquely to the nerve axis. Between these collagen bundles, a very coarse meshwork of randomly oriented collagen fibrils was present. In the perineurium, collagen fibrils occupied the interspaces between the concentrically arranged perineurial cells; in each interspace, they formed a sheet of characteristic lacework elaborately interwoven by thin (about 3 microns or less in width) bundles of collagen fibrils. In the subperineurial region, there was a distinct sheet of densely woven collagen fibrils between the perineurium and underlying endoneurial fibroblasts. In the endoneurium, collagen fibrils surrounded individual nerve fibers in two layers as scaffolds: the inner layer was made up of a delicate meshwork of very fine collagen fibrils, and the outer one consisted of longitudinally oriented bundles of about 1-3 microns in width. The collagen fibril arrangement described above may protect the nerve fibers against external forces.
通过氢氧化钠处理消化细胞成分后，利用扫描电子显微镜以及传统透射电子显微镜对大鼠坐骨神经中胶原纤维的组织结构进行了研究。神经外膜主要由宽度约为10 - 20微米的粗大胶原纤维束组成；它们呈波浪状，与神经轴略有倾斜。在这些胶原纤维束之间，存在着由随机取向的胶原纤维构成的非常粗糙的网络。在神经束膜中，胶原纤维占据了同心排列的神经束膜细胞之间的间隙；在每个间隙中，它们形成了一片由细（宽度约3微米或更小）胶原纤维束精心交织而成的独特花边状结构。在神经束膜下区域，在神经束膜和下方的神经内膜成纤维细胞之间有一片明显的紧密编织的胶原纤维。在神经内膜中，胶原纤维以两层支架的形式围绕着单个神经纤维：内层由非常细的胶原纤维构成的精细网络组成，外层由宽度约为1 - 3微米的纵向排列的纤维束组成。上述胶原纤维的排列方式可能保护神经纤维免受外力影响。
