Sedova V M, Vorob'ev V I
Biokhimiia. 1993 Oct;58(10):1580-6.
The procedure for isolation and purification of RNA polymerase II from human placenta nuclei is described. The sensitivity of the enzyme to alpha-amanitin, bivalent cations, ionic strength and glycerol concentration in vitro has been studied. Eleven subunits of the RNA polymerase II molecule have been identified by gel electrophoresis. An optimal RNA polymerase II assay mixture has been developed.
