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Characterization of RNA polymerase type II from human term placenta.

作者信息

Freund E, McGuire P M

出版信息

J Cell Physiol. 1986 Jun;127(3):432-8. doi: 10.1002/jcp.1041270312.

DOI:10.1002/jcp.1041270312
PMID:3754875
Abstract

RNA polymerase type II from human term placenta has been isolated and characterized with respect to its template, ammonium sulfate, divalent cation, and buffer preferences. In addition, the apparent Michaelis constants for AMP and UMP incorporation have been determined. The enzyme was also analyzed by native and denaturing polyacrylamide gel electrophoresis, and evidence is presented that a single polypeptide is radiolabeled with azido purine nucleoside triphosphate photoprobes.

摘要

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引用本文的文献

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Six human RNA polymerase subunits functionally substitute for their yeast counterparts.六种人类RNA聚合酶亚基在功能上可替代其酵母对应物。
Mol Cell Biol. 1995 Dec;15(12):6895-900. doi: 10.1128/MCB.15.12.6895.
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Functional substitution of an essential yeast RNA polymerase subunit by a highly conserved mammalian counterpart.
一个高度保守的哺乳动物对应物对酵母必需RNA聚合酶亚基的功能替代。
Mol Cell Biol. 1994 Jun;14(6):4155-9. doi: 10.1128/mcb.14.6.4155-4159.1994.