Kubo O, Kamijo Y, Himuro H, Kitamura K
No Shinkei Geka. 1976 Dec;4(12):1149-55.
10 acoustic neurinomas, 2 spinal cord neurinomas and 3 neurofibromas of von Recklinghausen's disease were elaborately studied by monolayer tissue culture method and Gelfoam organ culture method. Four morphological types were found in the acoustic and spinal cord neurinoma. (1) multinucleated cells (type I), dividing into bipolar spindle-shaped cells. (2) bipolar spindle-shaped cells (type II), forming of palisade fashion in the monolayer tissue culture method. (3) fibroblastic cells (type III), tending to predominate in the long-term cultures. (4) round cells (type IV), gathering to the Gelfoam mesh in the organ culture method. Neurofibroma of von Recklinghausen's disease was divided into cells of two types in the tissue culture. (1) spindle-shaped cells, rarely observed, and have been thinking that the cells originated from schwann cells. (2) round cells, having the macrophagic character, are predominant in the long-term culture.
采用单层组织培养法和明胶海绵器官培养法，对10例听神经瘤、2例脊髓神经瘤和3例冯雷克林霍增氏病神经纤维瘤进行了详细研究。在听神经瘤和脊髓神经瘤中发现了四种形态类型。（1）多核细胞（I型），可分化为双极梭形细胞。（2）双极梭形细胞（II型），在单层组织培养法中呈栅栏状排列。（3）成纤维细胞（III型），在长期培养中倾向于占主导地位。（4）圆形细胞（IV型），在器官培养法中聚集在明胶海绵网中。冯雷克林霍增氏病神经纤维瘤在组织培养中分为两种类型的细胞。（1）梭形细胞，很少观察到，一直认为这些细胞起源于施万细胞。（2）圆形细胞，具有巨噬细胞特征，在长期培养中占主导地位。
