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利用银增强试剂盒对地中海条纹海豚(条纹原海豚)肝脏、肾脏、肺和大脑中汞 - 硒相互作用产物进行微观定位

Microscopic localization of mercury-selenium interaction products in liver, kidney, lung and brain of Mediterranean striped dolphins (Stenella Coeruleoalba) by silver enhancement kit.

作者信息

Augier H, Benkoël L, Brisse J, Chamlian A, Park W K

机构信息

Centre d'Etudes, de Recherches et d'Informations sur la Mer (CERIMER), Faculté des Sciences de Luminy, Marseille, France.

出版信息

Cell Mol Biol (Noisy-le-grand). 1993 Nov;39(7):765-72.

PMID:8268761
Abstract

Microscopic observation, using physical development of silver, was carried out to localize the mercury-selenium interaction products in the organs of Mediterranean striped dolphins. The silver-metal reaction products were located mainly in hepatocytes and macrophages for liver, in proximal tubules for kidney. They were less abundant in lung than in liver and kidney. The result of semi-quantitative histochemistry tests showed that silver staining deposits were more abundant at relatively high metal concentrations than low metal contents, but independent of the metal contents. Comparisons with the most concentrated metal contents suggested that there might be a new complex of mercury and selenium, which could not be stainable by physical silver development.

摘要

利用银的物理显影进行显微镜观察,以定位地中海条纹海豚器官中的汞 - 硒相互作用产物。银 - 金属反应产物主要位于肝脏的肝细胞和巨噬细胞中,在肾脏的近端小管中。它们在肺中的含量低于肝脏和肾脏。半定量组织化学测试结果表明,银染色沉积物在相对高的金属浓度下比低金属含量时更丰富,但与金属含量无关。与最高浓度的金属含量相比表明,可能存在一种新的汞和硒复合物,其不能通过物理银显影进行染色。

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