Palmer S M, Gilkeson G S, Pisetsky D S
Division of Rheumatology and Immunology, Durham Veterans Administration Hospital, NC.
Lupus. 1993 Aug;2(4):251-5. doi: 10.1177/096120339300200408.
Immunization of normal mice with bacterial DNA elicits a significant IgG anti-DNA response and has been explored as a model of systemic lupus erythematosus. To determine whether this induced response is influenced by sex, we have measured anti-DNA levels in normal male and female BALB/c mice immunized with single stranded DNA from E. coli as complexes with methylated bovine serum albumin (mBSA) in adjuvant. By ELISA assays, anti-DNA levels of immunized females were approximately 16-fold higher than those of immunized males; levels of antibodies to the mBSA carrier were similar, however. The antibodies from females and males showed a similar degree of cross-reactivity when assayed using other natural and synthetic DNA antigens, including mammalian DNA. These findings suggest the potentiation of anti-DNA production in females by antigen-specific mechanisms and provide further evidence that immunization with bacterial DNA replicates features of autoantibody production in SLE.
用细菌DNA对正常小鼠进行免疫会引发显著的IgG抗DNA反应，并且已被作为系统性红斑狼疮的模型进行研究。为了确定这种诱导反应是否受性别影响，我们测量了用大肠杆菌单链DNA与甲基化牛血清白蛋白（mBSA）在佐剂中形成的复合物免疫的正常雄性和雌性BALB/c小鼠的抗DNA水平。通过ELISA检测，免疫雌性小鼠的抗DNA水平比免疫雄性小鼠高约16倍；然而，针对mBSA载体的抗体水平相似。当使用其他天然和合成DNA抗原（包括哺乳动物DNA）进行检测时，来自雌性和雄性的抗体表现出相似程度的交叉反应性。这些发现表明，抗原特异性机制可增强雌性小鼠抗DNA的产生，并进一步证明用细菌DNA免疫可重现系统性红斑狼疮中自身抗体产生的特征。
