Huang T H, Quesenberry J T, Martin M B, Loy T S, Diaz-Arias A A
Department of Pathology, Ellis Fischel Cancer Center, Columbia, Missouri.
Diagn Mol Pathol. 1993 Jun;2(2):90-3.
We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed microsatellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.
我们利用微卫星多态性，在福尔马林固定、石蜡包埋的结直肠癌组织中测定杂合性缺失。该多态性通过聚合酶链反应（PCR）进行DNA扩增来检测。采用PCR分析的微卫星方法，对从结肠癌石蜡包埋块中提取的降解DNA进行检测。使用位于结直肠癌基因内含子内的二核苷酸微卫星，成功扩增了26个肿瘤及其相应正常组织样本的DNA。在33%的信息性结直肠癌中检测到该标记上的等位基因缺失。本研究表明，微卫星为存档标本的杂合性缺失提供了一组强大的DNA标记。
