Lukácová M, Kazár J, Gajdosová E, Vavreková M
Institute of Virology, Slovak Academy of Sciences, Bratislava.
FEMS Microbiol Lett. 1993 Nov 1;113(3):285-9. doi: 10.1111/j.1574-6968.1993.tb06528.x.
Changes of lipopolysaccharide (LPS) of Coxiella burnetii strain Priscilla during chick embryo yolk sac passaging were observed by SDS-PAGE and immunoblot analysis. The course of LPS phase variation was similar to that found in other C. burnetti strains, i.e. a conversion of the phase I to the intermediate phase II after 10 passages. The intermediate phase II LPS of Priscilla strain was also detectable by immunoblot analysis using immune serum against Priscilla strain in the 30th passage.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹分析观察了贝氏柯克斯体普里西拉菌株在鸡胚卵黄囊传代过程中脂多糖（LPS）的变化。LPS相变过程与在其他贝氏柯克斯体菌株中发现的过程相似，即在传代10次后，I相转变为中间II相。使用针对第30代普里西拉菌株的免疫血清进行免疫印迹分析，也可检测到普里西拉菌株的中间II相LPS。
