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Human complement serine proteases C1r and C1s and their proenzymes.

作者信息

Arlaud G J, Thielens N M

机构信息

Institut de Biologie Structurale, Laboratoire d'Enzymologie Moléculaire, Grenoble, France.

出版信息

Methods Enzymol. 1993;223:61-82. doi: 10.1016/0076-6879(93)23038-o.

DOI:10.1016/0076-6879(93)23038-o
PMID:8271968
Abstract
摘要

相似文献

1
Human complement serine proteases C1r and C1s and their proenzymes.人补体丝氨酸蛋白酶C1r和C1s及其酶原。
Methods Enzymol. 1993;223:61-82. doi: 10.1016/0076-6879(93)23038-o.
2
Isolation of human complement subcomponents C1r and C1s in their unactivated, proenzyme forms.以未激活的酶原形式分离人补体亚成分C1r和C1s。
J Immunol Methods. 1991 Aug 9;141(2):219-26. doi: 10.1016/0022-1759(91)90148-9.
3
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.一种使用快速蛋白质色谱法以酶原形式纯化补体亚成分C1r和C1s的快速高效方法。
J Immunol Methods. 1988 Apr 6;108(1-2):265-9. doi: 10.1016/0022-1759(88)90428-0.
4
Activation of human complement serine-proteinase C1r is down-regulated by a Ca(2+)-dependent intramolecular control that is released in the C1 complex through a signal transmitted by C1q.人补体丝氨酸蛋白酶C1r的激活通过一种Ca(2+)依赖性分子内调控被下调,这种调控在C1复合物中通过C1q传递的信号被释放。
Biochem J. 1994 Jul 15;301 ( Pt 2)(Pt 2):509-16. doi: 10.1042/bj3010509.
5
Recombinant human complement subcomponent C1s lacking beta-hydroxyasparagine, sialic acid, and one of its two carbohydrate chains still reassembles with C1q and C1r to form a functional C1 complex.缺乏β-羟基天冬酰胺、唾液酸及其两条碳水化合物链之一的重组人补体亚成分C1s仍能与C1q和C1r重新组装形成功能性C1复合物。
Biochemistry. 1992 May 5;31(17):4254-62. doi: 10.1021/bi00132a015.
6
The cleavage of two C1s subunits by a single active C1r reveals substantial flexibility of the C1s-C1r-C1r-C1s tetramer in the C1 complex.单个活性C1r对两个C1s亚基的切割揭示了C1复合物中C1s - C1r - C1r - C1s四聚体具有相当大的灵活性。
J Immunol. 2000 Aug 15;165(4):2048-51. doi: 10.4049/jimmunol.165.4.2048.
7
Limited proteolysis of beta 2-microglobulin at Lys-58 by complement component C1s.补体成分C1s对β2-微球蛋白在赖氨酸58处的有限蛋白水解作用。
Eur J Biochem. 1990 Apr 30;189(2):423-9. doi: 10.1111/j.1432-1033.1990.tb15505.x.
8
Early complement proteases: C1r, C1s and MASPs. A structural insight into activation and functions.早期补体蛋白酶:C1r、C1s和甘露聚糖结合凝集素相关丝氨酸蛋白酶。对激活和功能的结构洞察。
Mol Immunol. 2009 Sep;46(14):2745-52. doi: 10.1016/j.molimm.2009.04.026. Epub 2009 May 27.
9
Classical complement pathway components C1r and C1s: purification from human serum and in recombinant form and functional characterization.经典补体途径成分C1r和C1s:从人血清中纯化及重组形式的制备与功能特性研究
Methods Mol Biol. 2014;1100:43-60. doi: 10.1007/978-1-62703-724-2_4.
10
Structure and function of the serine-protease subcomponents of C1: protein engineering studies.补体C1丝氨酸蛋白酶亚成分的结构与功能:蛋白质工程研究
Immunobiology. 1998 Aug;199(2):317-26. doi: 10.1016/S0171-2985(98)80036-3.

引用本文的文献

1
Complement C1q Interacts With LRP1 Clusters II and IV Through a Site Close but Different From the Binding Site of Its C1r and C1s-Associated Proteases.补体 C1q 通过一个接近但不同于其 C1r 和 C1s 相关蛋白酶结合位点的位点与 LRP1 簇 II 和 IV 相互作用。
Front Immunol. 2020 Oct 21;11:583754. doi: 10.3389/fimmu.2020.583754. eCollection 2020.
2
Mapping surface accessibility of the C1r/C1s tetramer by chemical modification and mass spectrometry provides new insights into assembly of the human C1 complex.通过化学修饰和质谱法绘制 C1r/C1s 四聚体的表面可及性图谱,为研究人 C1 复合物的组装提供了新的见解。
J Biol Chem. 2010 Oct 15;285(42):32251-63. doi: 10.1074/jbc.M110.149112. Epub 2010 Jun 30.
3
Calcium-dependent conformational flexibility of a CUB domain controls activation of the complement serine protease C1r.
钙离子依赖的 CUB 结构域构象灵活性控制补体丝氨酸蛋白酶 C1r 的激活。
J Biol Chem. 2010 Apr 16;285(16):11863-9. doi: 10.1074/jbc.M109.098541. Epub 2010 Feb 23.
4
Crystal structure of the catalytic domain of human complement c1s: a serine protease with a handle.人补体C1s催化结构域的晶体结构:一种带有柄的丝氨酸蛋白酶。
EMBO J. 2000 Apr 17;19(8):1755-65. doi: 10.1093/emboj/19.8.1755.