Helm R, Cockrell G, Sharkey P, Brenner R, Burks A W
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72202.
Mol Immunol. 1993 Dec;30(18):1685-8. doi: 10.1016/0161-5890(93)90443-f.
The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD. SDS-PAGE/Western blotting of non-radiolabeled translation products and incubation with human serum with IgE to cockroach allergens showed the presence of a 36 kD and 50 kD allergen. The confirmation of the translation of the cockroach allergens from total RNA is an important first step in the cloning of cockroach allergens.
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