Arruda L K, Vailes L D, Benjamin D C, Chapman M D
Asthma and Allergic Disease Center, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):295-7. doi: 10.1159/000237006.
Allergens produced by cockroaches (CRs) are an important cause of IgE antibody responses and asthma. Using molecular cloning and nucleic acid hybridization techniques, we have identified and sequenced several important allergens produced by the German CR (Blattella germanica) and studied their expression in the American CR (Periplaneta americana). Principal allergens include Bla g 2 (36-kD protein) and Bla g 4 (21-kD protein), to which 60-70% of CR-allergic patients make IgE antibodies. Bla g 2 is only expressed by B. germanica, whereas DNA encoding Bla g 4 is present in P. americana, but is not transcribed into mRNA. Sequence homology searches have identified Bla g 2 as an aspartic protease and Bla g 4 as a calycin. Other CR allergens that have been cloned include a glutathione transferase and a troponin. These studies will enable recombinant allergens to be expressed and used to investigate the role of CR allergens in asthma.
蟑螂产生的过敏原是导致IgE抗体反应和哮喘的重要原因。利用分子克隆和核酸杂交技术，我们已鉴定并测序了德国小蠊（德国小蠊）产生的几种重要过敏原，并研究了它们在美国蟑螂（美洲大蠊）中的表达。主要过敏原包括Bla g 2（36-kD蛋白）和Bla g 4（21-kD蛋白），60-70%的对蟑螂过敏的患者会针对这两种过敏原产生IgE抗体。Bla g 2仅由德国小蠊表达，而编码Bla g 4的DNA存在于美洲大蠊中，但不会转录成mRNA。序列同源性搜索已确定Bla g 2为天冬氨酸蛋白酶，Bla g 4为亲环素。其他已克隆的蟑螂过敏原包括谷胱甘肽转移酶和肌钙蛋白。这些研究将使重组过敏原得以表达，并用于研究蟑螂过敏原在哮喘中的作用。
