Helm R M, Squillace D L, Jones R T, Brenner R J
Department of Immunology, Mayo Medical School, Mayo Clinic, Rochester, Minn.
Int Arch Allergy Appl Immunol. 1990;92(2):154-61. doi: 10.1159/000235207.
Whole-body extracts of the feral and peridomestic Asian cockroach (Blattella asahinai) and the three domestic cockroach species, German (Blattella germanica), American (Periplaneta americana), and Oriental (Blatta orientalis), were compared allergenically using an IgE serum pool from 4 German cockroach sensitive individuals. In crossover radioallergosorbent inhibition analysis, the Asian cockroach shared allergenic activity primarily with the German cockroach polymer and to a lesser extent with either the American or Oriental cockroach polymers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and thin-layer isoelectric focusing analysis of the extracts showed similar although varying intensities of Coomassie blue stained banding patterns among five extracts analyzed. Electroblotting analysis with 12.5% SDS-PAGE of the whole-body German cockroach extract and IgE serum from individuals sensitive to German cockroach revealed eight allergenic components with apparent molecular weights of 92, 80, 67, 48, 36, 27, 25 and 18 kD. Five components could be identified in the whole-body extract of the Asian cockroach corresponding to apparent molecular weights of 92, 67, 48, 40, and 32 kD. Analysis of individual serum by immunoblot analysis with each of the cockroach extracts showed considerable heterogenicity in the IgE-binding pattern. Although the Asian cockroach demonstrated considerable cross-reacting allergenic components to German and relatively fewer cross-reacting allergenic components to either the Oriental or American, it is too early to establish genus- or species-specific cockroach allergens. It is important to point out that German cockroach sensitive individuals should be made aware of the potential exposure of Asian cockroach aeroallergens both indoors and outdoors in areas with high infestations of Asian cockroaches.
利用来自4名对德国小蠊敏感个体的IgE血清池，比较了野生和周边地区亚洲蜚蠊（日本姬蠊）以及三种家栖蜚蠊物种，即德国小蠊、美洲大蠊和东方蜚蠊的全身提取物的变应原性。在交叉放射变应原吸附抑制分析中，亚洲蜚蠊主要与德国小蠊聚合物具有共同变应原活性，与美洲大蠊或东方蜚蠊聚合物的共同变应原活性较低。提取物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和薄层等电聚焦分析显示，在所分析的五种提取物中，考马斯亮蓝染色条带模式相似，但强度各异。对德国小蠊全身提取物和对德国小蠊敏感个体血清进行12.5% SDS-PAGE的电印迹分析显示，有8种变应原成分，其表观分子量分别为92、80、67、48、36、27、25和18 kD。在亚洲蜚蠊全身提取物中可鉴定出5种成分，其表观分子量分别为92、67、48、40和32 kD。用每种蜚蠊提取物通过免疫印迹分析对个体血清进行分析显示，IgE结合模式存在相当大的异质性。尽管亚洲蜚蠊对德国小蠊显示出相当多的交叉反应性变应原成分，对东方蜚蠊或美洲大蠊的交叉反应性变应原成分相对较少，但确定属或种特异性蜚蠊变应原还为时过早。需要指出的是，在亚洲蜚蠊大量滋生的地区室内和室外环境中接触亚洲蜚蠊气传变应原的可能性，应该让对德国小蠊敏感的个体知晓。
