Hulkower K I, Coffey J W, Levin W, Anderson C M, Chen T, Hope W C, Bolin D R, Morgan D W
Department of Bronchopulmonary Research, Hoffmann-La Roche Inc., Nutley, NJ 07110.
Agents Actions. 1993;39 Spec No:C5-7. doi: 10.1007/BF01972703.
We investigated the temporal relationship between the increase in enzymatic activity and protein of a high molecular weight (100 kDa), cytosolic PLA2 (cPLA2) in interleukin-1 beta (IL-1 beta)-treated rheumatoid synovial fibroblasts (RSF). Both of these responses increased according to a similar time-course which correlates with PGE2 production by these cells. In contrast, 14 kDa, secreted PLA2 (sPLA2), which was also produced by RSF, was not affected by IL-1 beta treatment. These findings support that an augmentation of CPLA2 activity, caused by an induction of cPLA2 protein, rather than sPLA2, is temporally associated with increased PGE2 production in IL-1 beta-treated RSF.
我们研究了白细胞介素-1β（IL-1β）处理的类风湿性滑膜成纤维细胞（RSF）中，高分子量（100 kDa）胞质型磷脂酶A2（cPLA2）的酶活性增加与蛋白质增加之间的时间关系。这两种反应均按照与这些细胞产生前列腺素E2（PGE2）相关的相似时间进程增加。相比之下，RSF也产生的14 kDa分泌型磷脂酶A2（sPLA2）不受IL-1β处理的影响。这些发现支持，由cPLA2蛋白诱导而非sPLA2引起的cPLA2活性增强在时间上与IL-1β处理的RSF中PGE2产生增加相关。
