[绿蛙（食用蛙）中的促黑素：生化与放射免疫研究]

[Melanotropic hormone in the green frog (Rana esculenta): biochemical and radioimmunological study].

作者信息

Vaudry H, Oliver C, Usategui R, Trochard M C, Lwboulenger F, Dupont W, Vaillant R

出版信息

C R Acad Hebd Seances Acad Sci D. 1976 Dec 8;283(15):1655-8.

Abstract

The alpha-MSH levels in the frog hypophysis were measured by use of a sensitive ans specific radioimmunoassay. Sephadex G-50 gel chromatography of frog pars distalis and pars intermedia extracts showed that frog hypophyseal MSH was chemically related to synthetic alpha-MSH. Rat tissue homogenates were capable of degrading frog pars intermedia MSH as well as synthetic alpha-MSH. These results provide evidence that the frog hypophysis--pars intermedia and pars distalis--contains an alpha-MSH-like peptide.

摘要

采用灵敏且特异的放射免疫分析法测定了蛙脑垂体中的α-促黑素（α-MSH）水平。对蛙远侧部和中间部提取物进行葡聚糖凝胶G-50柱层析分析表明，蛙脑垂体促黑素在化学性质上与合成的α-促黑素相关。大鼠组织匀浆能够降解蛙中间部促黑素以及合成的α-促黑素。这些结果证明，蛙脑垂体——中间部和远侧部——含有一种α-促黑素样肽。