Jégou S, Tonon M C, Leroux P, Delarue C, Leboulenger F, Pelletier G, Côté J, Ling N, Vaudry H
Gen Comp Endocrinol. 1983 Aug;51(2):246-54. doi: 10.1016/0016-6480(83)90078-3.
To gain more information about the nature and regulation of opiomelanocorticotropic peptides in the frog diencephalon, radioimmunological determinations of alpha- and beta-MSH. ACTH, beta- and gamma-endorphins have been performed in hypothalamic extracts. Sephadex G-50 gel filtration revealed a single peak of alpha-MSH-like immunoreactivity (alpha-MSH-LI) comigrating with synthetic alpha-MSH. Two peaks of ACTH-LI were observed; the major one eluting slightly before human ACTH. Using a porcine beta-endorphin antiserum which exhibited 45% cross-reaction (on a molar basis) with ovine beta-LPH, one major peak of beta-endorphin-LI and two additional components were observed in the elution profile; none of these peaks coeluted with synthetic porcine beta-endorphin. No significant beta-MSH or gamma-endorphin-LI was detected. To investigate a possible role of glucocorticoids on the level of opiomelanocorticotropic peptides in frog hypothalamus, 60 male frogs were treated with dexamethasone (300 micrograms/day) during 8 days. Dexamethasone treatment did not modify the chromatographic distribution and the total amount of alpha-MSH-LI and ACTH-LI in hypothalamic extracts. A slight (15%) increase in beta-endorphin-LI was even observed after 8 days of dexamethasone treatment. From these results it is concluded that three classes of opiomelanocorticotropic peptides are present in the frog hypothalamus in the following order of concentration: beta-endorphin-LI greater than alpha-MSH greater than ACTH. In addition, circulating glucocorticoids which significantly reduce the concentration of opiomelanocorticotropic hormones in the distal lobe of the frog pituitary (S. Jégou, M. C. Tonon, F. Leboulenger, C. Delarue, J. Côté, G. Pelletier, and H. Vaudry (1981a). In "Adv. Physiol. Sci.' E. Stark, G. B. Makara, Zs. Acs, and E. Endröczi, eds., Vol. 13, pp. 129-133. Pergamon, Budapest.) do not modify the amount of these peptides in the hypothalamus.
为了获取更多关于蛙类间脑中阿片黑素皮质激素肽的性质和调节的信息，已对下丘脑提取物中的α-和β-促黑素细胞激素（MSH）、促肾上腺皮质激素（ACTH）、β-和γ-内啡肽进行了放射免疫测定。葡聚糖凝胶G-50柱层析显示，与合成α-MSH共同迁移的α-MSH样免疫反应性（α-MSH-LI）有一个单一峰。观察到ACTH-LI有两个峰；主要的一个峰在人ACTH之前稍有洗脱。使用与绵羊β-促脂素（β-LPH）有45%交叉反应（基于摩尔比）的猪β-内啡肽抗血清，在洗脱图谱中观察到一个主要的β-内啡肽-LI峰和另外两个成分；这些峰均未与合成猪β-内啡肽共洗脱。未检测到显著的β-MSH或γ-内啡肽-LI。为了研究糖皮质激素对蛙下丘脑阿片黑素皮质激素肽水平的可能作用，60只雄性蛙在8天内用 dexamethasone（300微克/天）进行处理。dexamethasone处理未改变下丘脑提取物中α-MSH-LI和ACTH-LI的色谱分布及总量。甚至在dexamethasone处理8天后观察到β-内啡肽-LI略有增加（15%）。从这些结果可以得出结论，蛙下丘脑存在三类阿片黑素皮质激素肽，其浓度顺序如下：β-内啡肽-LI＞α-MSH＞ACTH。此外，循环糖皮质激素能显著降低蛙垂体远侧部阿片黑素皮质激素的浓度（S. Jégou、M. C. Tonon、F. Leboulenger、C. Delarue、J. Côté、G. Pelletier和H. Vaudry（1981a）。载于《生理学进展》，E. Stark、G. B. Makara、Zs. Acs和E. Endröczi编，第13卷，第129 - 133页。Pergamon出版社，布达佩斯。），但不改变下丘脑这些肽的含量。
