家禽中的高度多态微卫星标记

Highly polymorphic microsatellite markers in poultry.

作者信息

Crooijmans R P, van Kampen A J, van der Poel J J, Groenen M A

机构信息

Department of Animal Breeding, Agricultural University Wageningen, The Netherlands.

出版信息

Anim Genet. 1993 Dec;24(6):441-3. doi: 10.1111/j.1365-2052.1993.tb00360.x.

DOI:10.1111/j.1365-2052.1993.tb00360.x

PMID:8273919

Abstract

Microsatellite markers have been established for a large number of species, but up till now very few polymorphic microsatellite markers have been reported in poultry. We have isolated 34 polymorphic chicken microsatellite markers of the poly(TG) type. The number of repeats varied from 9 up to 33. Often, other repeats such as poly(T) or poly (GAA) were present adjacent to the poly (TG) repeat. Polymerase chain reaction amplification of the microsatellites resulted in detection of three or more alleles in a test panel of five different animals for 75% of the microsatellites. Segregation of five microsatellite markers has been tested in a small family.

摘要

微卫星标记已在大量物种中得到确立，但截至目前，在家禽中报道的多态微卫星标记却非常少。我们已分离出34个多态性的鸡多聚（TG）型微卫星标记。重复序列的数量从9个到33个不等。通常，多聚（TG）重复序列附近还存在其他重复序列，如多聚（T）或多聚（GAA）。对这些微卫星进行聚合酶链反应扩增后，在由五只不同动物组成的测试组中，75%的微卫星检测到三个或更多等位基因。已在一个小家族中对五个微卫星标记的分离情况进行了测试。