Khatib H, Genislav E, Crittenden L B, Bumstead N, Soller M
Department of Genetics, Hebrew University of Jerusalem, Israel.
Anim Genet. 1993 Oct;24(5):355-62. doi: 10.1111/j.1365-2052.1993.tb00340.x.
Two chicken genomic libraries were screened for the presence of poly(TG/AC) microsatellite tracts. The number of positive clones was low, confirming the low frequency of such microsatellites in the chicken genome relative to mammalian genomes. Polymorphism of 29 microsatellite tracts, comprising 11 from the library screening and 18 obtained from GenBank, was examined in the East Lansing and Compton reference families, in a resource population formed by a cross between a single White Rock broiler and inbred Leghorn females, and in a panel of birds from five layer stocks. Twenty microsatellites, primarily of the poly(TG/AC) type, were polymorphic in at least one of the populations. Thirteen of the microsatellites were polymorphic in the East Lansing reference family and 13 were also polymorphic in the resource population, confirming that the genetic distance between White Rock and White Leghorn is about as great as between Jungle fowl and White Leghorn. Only six microsatellites were polymorphic in the Compton reference family, formed by a cross between two White Leghorn strains. Twelve of the microsatellites were mapped in the East Lansing and/or Compton reference families. These were well dispersed among the various linkage groups and did not show any indications of terminal clustering.
对两个鸡基因组文库进行筛选，以检测是否存在聚（TG/AC）微卫星序列。阳性克隆的数量很少，这证实了相对于哺乳动物基因组而言，此类微卫星在鸡基因组中的频率较低。在伊斯特兰辛和康普顿参考家系、由一只白洛克肉鸡与近交来航蛋鸡母本杂交形成的资源群体以及来自五个蛋鸡品系的一组鸡中，检测了29个微卫星序列的多态性，其中11个来自文库筛选，18个来自GenBank。20个微卫星，主要是聚（TG/AC）类型，在至少一个群体中具有多态性。13个微卫星在伊斯特兰辛参考家系中具有多态性，13个在资源群体中也具有多态性，这证实了白洛克鸡和白来航鸡之间的遗传距离与原鸡和白来航鸡之间的遗传距离大致相同。在由两个白来航鸡品系杂交形成的康普顿参考家系中，只有6个微卫星具有多态性。12个微卫星在伊斯特兰辛和/或康普顿参考家系中进行了定位。它们在各个连锁群中分布良好，没有显示出任何末端聚类的迹象。
