Casas C, Lacey C J, Meunier B
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Bioconjug Chem. 1993 Sep-Oct;4(5):366-71. doi: 10.1021/bc00023a011.
Methods are reported for conjugating a cationic tris(methylpyridiniumyl)porphyrin-manganese(III) complex to oligonucleotides. The DNA cleaver motif used in the present work is related to Mn-TMPyP, a highly efficient artificial endonuclease able to hydroxylate C-H bonds of DNA sugars at nanomolar concentrations. A general coupling method has been developed by using porphyrin precursors having a linker attached at the para position of the meso phenyl group of the macrocycle and terminating with an activated ester of 1-hydroxybenzotriazole. Coupling reactions were monitored by HPLC. Purification of metalloporphyrin-oligonucleotide conjugates was performed by HPLC and desalting on Bio-Gel P-2. The purity of conjugates was always above 95%.
报道了将阳离子三（甲基吡啶基）卟啉-锰（III）配合物与寡核苷酸偶联的方法。本研究中使用的DNA切割基序与Mn-TMPyP有关，Mn-TMPyP是一种高效的人工内切核酸酶，能够在纳摩尔浓度下使DNA糖的C-H键羟基化。通过使用在大环中卟啉前体的中位苯基对位连接有接头并以1-羟基苯并三唑的活化酯终止的卟啉前体，开发了一种通用的偶联方法。通过HPLC监测偶联反应。通过HPLC和在Bio-Gel P-2上脱盐对金属卟啉-寡核苷酸缀合物进行纯化。缀合物的纯度始终高于95%。
