Loktionova N A, Beniashvili D Sh, Sartania M S, Zabezhinski M A, Kazanova O I, Petrov A S, Likhachev A J
Laboratory of Environmental Oncology, NN Petrov Research Institute of Oncology, St Petersburg, Russian Federation.
Biochimie. 1993;75(9):821-4. doi: 10.1016/0300-9084(93)90133-d.
The activity of a DNA repair enzyme, O6-alkylguanine-DNA alkyltransferase (AGT), was studied in gastric mucosa of 15 Macaca fascicularis monkeys before and during chronic oral exposure to the ethylating carcinogen N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) in order to investigate possible causes of inter-individual differences in susceptibility to its gastrocarcinogenic effect. A wide range of AGT activity (307-1903 fmol/mg protein, mean 695) was found before treatment and it decreased during the first year of exposure (means 627, 479 and 452 fmol/mg protein respectively at 6, 12 and 18 months after the beginning of the experiment). The carcinogenesis study is under way and to date four monkeys with low initial AGT level in gastric mucosa died of gastric cancer. The relevance of AGT level measurement for prediction of individual susceptibility to ENNG is discussed.
为了探究个体对乙基化致癌物N-乙基-N'-硝基-N-亚硝基胍（ENNG）胃致癌作用易感性存在个体差异的可能原因，对15只食蟹猴在长期口服ENNG之前和期间的胃黏膜中DNA修复酶O6-烷基鸟嘌呤-DNA烷基转移酶（AGT）的活性进行了研究。在治疗前发现AGT活性范围很广（307-1903 fmol/mg蛋白质，平均695），并且在接触的第一年中降低（在实验开始后的6、12和18个月时，AGT活性分别平均为627、479和452 fmol/mg蛋白质）。致癌作用研究正在进行中，迄今为止，胃黏膜中初始AGT水平较低的4只猴子死于胃癌。讨论了AGT水平测量对于预测个体对ENNG易感性的相关性。
