Influence of glucose on the deoxyglucose metabolism in S cerevisiae: detection and identification of deoxyglucose and trehalose derivatives by 1H- and 13C-NMR spectroscopy.

The metabolism of 2-deoxy-D-glucose (DG) in glucose grown repressed Saccharomyces cerevisiae cells was studied in the absence and presence of glucose (Glc) at 30 degrees C in a standard pyrophosphate medium containing 4.5 x 10(7) cells/ml. 1H- and 13C-NMR spectroscopy were successfully used to distinguish and identify several derivatives of DG and trehalose. Using [1-13C]DG, alpha- and beta-DG, alpha- and beta-DG6P, dideoxy-trehalose (DG-DG) and deoxy-trehalose (DG-Glc) can be simultaneously observed in the intracellular medium. The [DG6P]/[DG] ratio is about 5-6. The results seem to indicate the existence of an equilibrium between DG and DG6P, which limits the production of DG6P in cells. Glucose was found to exert a great influence on the metabolism of DG. It favours the formation of DG-DG and DG-Glc.