Strong T V, Tagle D A, Valdes J M, Elmer L W, Boehm K, Swaroop M, Kaatz K W, Collins F S, Albin R L
Department of Internal Medicine, University of Michigan, Ann Arbor 48109.
Nat Genet. 1993 Nov;5(3):259-65. doi: 10.1038/ng1193-259.
We have used RNA in situ hybridization to study the regional expression of the Huntington's disease gene (HD) and its rat homologue in brain and selected nonneural tissues. The HD transcript was expressed throughout the brain in both rat and human, especially in the neurons of the dentate gyrus and pyramidal neurons of the hippocampal formation, cerebellar granule cell layer, cerebellar Purkinje cells and pontine nuclei. Other brain areas expressed lower levels of the HD transcript without pronounced regional differences. Neuronal expression predominated over glial expression in all regions. HD mRNA was also expressed in colon, liver, pancreas and testes. The regional specificity of neuropathology in HD, which is most prominent in the basal ganglia, thus cannot be accounted for by the pattern of expression of HD.
我们运用RNA原位杂交技术研究了亨廷顿舞蹈病基因（HD）及其大鼠同源基因在脑和选定的非神经组织中的区域表达情况。HD转录本在大鼠和人类的整个脑中均有表达，尤其是在齿状回的神经元、海马结构的锥体细胞、小脑颗粒细胞层、小脑浦肯野细胞和脑桥核中。其他脑区HD转录本表达水平较低，无明显区域差异。在所有区域，神经元表达均高于胶质细胞表达。HD mRNA在结肠、肝脏、胰腺和睾丸中也有表达。因此，HD中神经病理学的区域特异性（在基底神经节中最为突出）无法用HD的表达模式来解释。
