Kormelink F J, Gruppen H, Voragen A G
Department of Food Science, Agricultural University, Biotechnion, Wageningen, Netherlands.
Carbohydr Res. 1993 Nov 3;249(2):345-53. doi: 10.1016/0008-6215(93)84099-r.
Arabinoxylan-derived oligosaccharides were treated with (1-->4)-beta-D-arabinoxylan arabinofuranohydrolase (AXH) and two types of alpha-L-arabinofuranosidase, A and B. Analysis of reaction products by high performance anion-exchange chromatography indicated the removal of arabinofuranosyl groups from singly substituted xylopyranosyl residues. In addition, differences in the specificity of these enzymes towards the various differently substituted oligosaccharides were observed. 1H NMR spectroscopy and methylation analysis of alkali-extractable wheat-flour arabinoxylan treated with AXH confirmed the specificity of AXH towards (1-->3)-linked arabinofuranosyl groups on singly substituted xylopyranosyl residues. With these techniques, alpha-L-arabinofuranosidase B was found to cause minor changes in (1-->2)- and (1-->3)-linked arabinofuranosyl groups on doubly substituted xylopyranosyl residues.
将阿拉伯木聚糖衍生的低聚糖用（1→4）-β-D-阿拉伯木聚糖阿拉伯呋喃水解酶（AXH）和两种α-L-阿拉伯呋喃糖苷酶A和B进行处理。通过高效阴离子交换色谱法对反应产物进行分析，结果表明从单取代的木吡喃糖基残基上去除了阿拉伯呋喃糖基。此外，还观察到这些酶对各种不同取代的低聚糖的特异性存在差异。对经AXH处理的碱提取小麦粉阿拉伯木聚糖进行的1H NMR光谱分析和甲基化分析证实了AXH对单取代木吡喃糖基残基上（1→3）连接的阿拉伯呋喃糖基的特异性。通过这些技术发现，α-L-阿拉伯呋喃糖苷酶B会使双取代木吡喃糖基残基上（1→2）和（1→3）连接的阿拉伯呋喃糖基发生微小变化。
