唾液中抗变形链球菌抗体对龋齿活性及变形链球菌黏附的影响

[The effect of antibody to Streptococcus mutans in saliva on caries activity and S. mutans adhering].

作者信息

Luo Z L

机构信息

College of Stomatology, West China University of Medical Sciences, Chendu.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 1993 May;28(3):164-6, 191.

PMID:8275809

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was used for measuring IgA antibody to whole cell of Streptococcus mutans (serotype c) in saliva. 36 parotid salivary samples of human were collected from two groups: Caries free (CF) and caries sensitive (CS). The result shows that the IgA antibody to S. mutans in CF group was higher than those in CS group (P < 0.05). The saliva antibody was gained from the rabbits by injection with S. mutans (serotype c), and the adhesion of S. mutans--3H on the surface of hydroxylapatite beads treated by rabbit's saliva was measured. The results show that the saliva with immunity could inhibit the S. mutans to adhere on the HA beads (P < 0.05). It means saliva antibody may prevent caries through inhibition of S. mutans from adhesion.

摘要

采用间接酶联免疫吸附测定法（ELISA）检测唾液中变形链球菌（c血清型）全细胞的IgA抗体。从两组人群中收集了36份腮腺唾液样本：无龋组（CF）和龋敏感组（CS）。结果显示，CF组中针对变形链球菌的IgA抗体高于CS组（P<0.05）。通过向兔子注射变形链球菌（c血清型）获得唾液抗体，并检测变形链球菌-3H在经兔唾液处理的羟基磷灰石珠表面的黏附情况。结果表明，具有免疫性的唾液可抑制变形链球菌黏附于HA珠（P<0.05）。这意味着唾液抗体可能通过抑制变形链球菌的黏附来预防龋齿。