Benfey P N, Linstead P J, Roberts K, Schiefelbein J W, Hauser M T, Aeschbacher R A
Department of Biology, New York University, NY 10003.
Development. 1993 Sep;119(1):57-70. doi: 10.1242/dev.119.Supplement.57.
A genetic analysis of root development in Arabidopsis thaliana has identified mutants that have abnormal morphogenesis. Four of these root morphogenesis mutants show dramatic alterations in post-embryonic root development. The short-root mutation results in a change from indeterminate to determinate root growth and the loss of internal root cell layers. The cobra and lion's tail mutations cause abnormal root cell expansion which is conditional upon the rate of root growth. Expansion is greatest in the epidermal cells in cobra and in the stele cells in lion's tail. The sabre mutation causes abnormal cell expansion that is greatest in the root cortex cell layer and is independent of the root growth rate. The tissue-specific effects of these mutations were characterized with monoclonal antibodies and a transgenic marker line. Genetic combinations of the four mutants have provided insight into the regulation of growth and cell shape during Arabidopsis root development.
对拟南芥根发育的遗传分析已鉴定出形态发生异常的突变体。其中四个根形态发生突变体在胚后根发育中表现出显著变化。短根突变导致根生长从不定型变为定型，并丧失内部根细胞层。眼镜蛇和狮尾突变导致根细胞异常扩张，这取决于根的生长速率。在眼镜蛇突变体中，表皮细胞的扩张最大；在狮尾突变体中，中柱细胞的扩张最大。军刀突变导致异常的细胞扩张，在根皮层细胞层中最为明显，且与根生长速率无关。利用单克隆抗体和转基因标记系对这些突变的组织特异性效应进行了表征。这四个突变体的遗传组合为深入了解拟南芥根发育过程中的生长调控和细胞形态提供了线索。
