Nath N, Biswas S, Bhelwa A, Ittoop A, Biswal S
Department of Biochemistry, Nagpur University.
Indian J Biochem Biophys. 1993 Aug;30(4):244-6.
With a view to determining the role of diabetic serum factor (DSF) in the progression of membrane pathology, time-dependent preincubation effects of DSF on certain membrane-bound enzymes of normal polymorphonuclear leucocytes (PMNL) have been studied. DSF is found to cause significant decrements in the activities of Na+/K+ ATPase, PLC and AcE of PMNL on in vitro incubation for 60 min, while the effect on Ca2+/Mg2+ ATPase appears only on prolonged incubation with an initial hike in activity at 5 min. The implications of these results have been discussed.
为了确定糖尿病血清因子（DSF）在膜病理学进展中的作用，研究了DSF对正常多形核白细胞（PMNL）某些膜结合酶的时间依赖性预孵育效应。发现DSF在体外孵育60分钟时会导致PMNL的Na+/K+ ATPase、PLC和AcE活性显著下降，而对Ca2+/Mg2+ ATPase的影响仅在长时间孵育时出现，且在5分钟时活性先升高。讨论了这些结果的意义。
