Yoshinaga Y, Matsuno Y, Fujita S, Nakamura T, Kikuchi M, Shimosato Y, Hirohashi S
Pathology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1993 Nov;84(11):1150-8. doi: 10.1111/j.1349-7006.1993.tb02815.x.
Hepatocyte growth factor (HGF)/scatter factor (SF) is a multifunctional factor considered to be potentially involved in tissue regeneration, wound healing, embryogenesis, angiogenesis and cancer invasion. Here we examined immunohistochemically the distribution of HGF/SF in human tissues, including cancerous and inflammatory tissues, using anti-HGF antibody. HGF/SF accumulation was clearly detected in the extracellular matrix, particularly along the basement membrane, in cancerous and inflammatory tissues, but only a little was detected in normal tissues. HGF/SF is well known to have a strong affinity for heparin in vitro, and from the results of our immunohistochemical assay, we considered that HGF/SF was bound to heparin or heparan sulfate of the extracellular matrix and basement membrane. HGF/SF was well localized in cancerous and inflammatory lesions of human lung, liver and pancreas, and in apparently normal tissues of kidney, adrenal gland and pancreas obtained at autopsy. In lung, HGF/SF was localized along the basement membranes of cancer cell nests, in the extracellular matrix of the cancer cell surface, cancer stroma and tissues invaded by cancer, and the basement membranes of bronchial epithelium and capillary vessels in inflammatory stroma. Since HGF/SF makes some cancer cells more invasive in vitro, the accumulation of HGF/SF in cancerous tissue suggests that the invasiveness of some cancer cells may be increased by HGF/SF in vivo.
肝细胞生长因子（HGF）/分散因子（SF）是一种多功能因子，被认为可能参与组织再生、伤口愈合、胚胎发育、血管生成和癌症侵袭。在此，我们使用抗HGF抗体，通过免疫组织化学方法检测了HGF/SF在人体组织（包括癌组织和炎症组织）中的分布。在癌组织和炎症组织的细胞外基质中，尤其是沿着基底膜，明显检测到HGF/SF的积聚，但在正常组织中仅检测到少量。众所周知，HGF/SF在体外对肝素有很强的亲和力，根据我们免疫组织化学检测的结果，我们认为HGF/SF与细胞外基质和基底膜中的肝素或硫酸乙酰肝素结合。HGF/SF在人肺、肝和胰腺的癌性和炎性病变中以及在尸检获得的肾、肾上腺和胰腺的明显正常组织中定位良好。在肺中，HGF/SF定位于癌细胞巢的基底膜、癌细胞表面的细胞外基质、癌基质和癌侵袭的组织以及炎症基质中支气管上皮和毛细血管的基底膜。由于HGF/SF在体外会使一些癌细胞更具侵袭性，癌组织中HGF/SF的积聚表明体内某些癌细胞的侵袭性可能会因HGF/SF而增加。
