Steinmetz M, Kunst F, Dedonder R
Mol Gen Genet. 1976 Nov 17;148(3):281-5. doi: 10.1007/BF00332902.
The sacUh, amyB and pap mutations are identical with respect to their pleiotropic phenotype and their genetic location. Strains bearing these mutations overproduce several exocellular enzymes: alpha amylase, lavansucrase and proteases, they are poorly or not at all transformable and most of them are devoid of flagella. These mutations are tightly linked to the sacU- mutations by transformation and therefore lie between the hisA1 and gtaB290 markers. It is possible that the sacUh, amyB and pap mutations on one hand and the sacU- mutations on the other are two different classes of alterations of the same regulatory gene controlling the synthesis of some exocellular enzymes and several other cellular functions. Furthermore an amy- mutation, leading to the lack of alpha-amylase activity, was mapped between the lin2 and aroI906 markers which are not linked to the sacU locus.
SacUh、amyB和pap突变在其多效性表型和基因定位方面是相同的。携带这些突变的菌株会过量产生几种胞外酶：α淀粉酶、蔗糖酶和蛋白酶，它们很难转化或根本无法转化，并且大多数菌株没有鞭毛。通过转化，这些突变与sacU - 突变紧密连锁，因此位于hisA1和gtaB290标记之间。有可能一方面SacUh、amyB和pap突变，另一方面sacU - 突变是同一调控基因的两类不同改变，该调控基因控制某些胞外酶的合成以及其他几种细胞功能。此外，一个导致缺乏α淀粉酶活性的amy - 突变被定位在与sacU位点不连锁的lin2和aroI906标记之间。
