Center for Microbial Ecology and Department of Microbiology, Michigan State University, East Lansing, Michigan 48824.
Appl Environ Microbiol. 1992 Aug;58(8):2701-3. doi: 10.1128/aem.58.8.2701-2703.1992.
A Bacillus subtilis transconjugant with a Tn916 chromosomal insert was obtained through mating with Escherichia coli carrying the transposon as a plasmid insert. Actinomycetes were identified as frequent transposon recipients following the introduction of the B. subtilis transconjugant into a soil microcosm.
通过与携带转座子质粒插入物的大肠杆菌进行交配,获得了带有 Tn916 染色体插入物的枯草芽孢杆菌转导体。将枯草芽孢杆菌转导体引入土壤微宇宙后,鉴定出放线菌是转座子的频繁受体。