Kuge O, Hara-Kuge S, Orci L, Ravazzola M, Amherdt M, Tanigawa G, Wieland F T, Rothman J E
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
J Cell Biol. 1993 Dec;123(6 Pt 2):1727-34. doi: 10.1083/jcb.123.6.1727.
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.
编码外被体蛋白20-kD亚基ζ-COP的cDNA预测其为一种含有177个氨基酸残基的蛋白质,其序列与网格蛋白衔接复合体的亚基AP17和AP19相似。针对ζ-COP的多克隆抗体可阻断外被体蛋白与高尔基体膜的结合,并阻止COP包被小泡在高尔基池上的组装。与其他外被体蛋白亚基(β-、β'-、γ-和ε-COP)不同,ζ-COP以结合态和游离态两种形式存在。