Klink Vincent P, Sharma Keshav, Pant Shankar R, McNeece Brant, Niraula Prakash, Lawrence Gary W
a Department of Biological Sciences , Mississippi State University , Mississippi State , MS , USA.
b Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology , Mississippi State University , Mississippi State , MS , USA.
Plant Signal Behav. 2017 Feb;12(2):e1274481. doi: 10.1080/15592324.2016.1274481.
The term regulon has been coined in the genetic model plant Arabidopsis thaliana, denoting a structural and physiological defense apparatus defined genetically through the identification of the penetration (pen) mutants. The regulon is composed partially by the soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) syntaxin PEN1. PEN1 has homology to a Saccharomyces cerevisae gene that regulates a Secretion (Sec) protein, Suppressor of Sec 1 (Sso1p). The regulon is also composed of the β-glucosidase (PEN2) and an ATP binding cassette (ABC) transporter (PEN3). While important in inhibiting pathogen infection, limited observations have been made regarding the transcriptional regulation of regulon genes until now. Experiments made using the model agricultural Glycine max (soybean) have identified co-regulated gene expression of regulon components. The results explain the observation of hundreds of genes expressed specifically in the root cells undergoing the natural process of defense. Data regarding additional G. max genes functioning within the context of the regulon are presented here, including Sec 14, Sec 4 and Sec 23. Other examined G. max homologs of membrane fusion genes include an endosomal bromo domain-containing protein1 (Bro1), syntaxin6 (SYP6), SYP131, SYP71, SYP8, Bet1, coatomer epsilon (ϵ-COP), a coatomer zeta (ζ-COP) paralog and an ER to Golgi component (ERGIC) protein. Furthermore, the effectiveness of biochemical pathways that would function within the context of the regulon ave been examined, including xyloglucan xylosyltransferase (XXT), reticuline oxidase (RO) and galactinol synthase (GS). The experiments have unveiled the importance of the regulon during defense in the root and show how the deposition of callose relates to the process.
“调控子”这一术语是在模式植物拟南芥中提出的，它指的是一种通过鉴定渗透（pen）突变体在基因层面定义的结构和生理防御机制。该调控子部分由可溶性N - 乙基马来酰亚胺敏感融合蛋白附着蛋白受体（SNARE） syntaxin PEN1组成。PEN1与酿酒酵母中一个调控分泌（Sec）蛋白Sec 1抑制因子（Sso1p）的基因具有同源性。该调控子还由β - 葡萄糖苷酶（PEN2）和一个ATP结合盒（ABC）转运蛋白（PEN3）组成。虽然在抑制病原体感染方面很重要，但到目前为止，关于调控子基因的转录调控的观察还很有限。利用模式农作物大豆进行的实验已经确定了调控子组分的共调控基因表达。这些结果解释了在经历自然防御过程的根细胞中特异性表达的数百个基因的观察结果。本文展示了在调控子背景下发挥作用的其他大豆基因的数据，包括Sec 14、Sec 4和Sec 23。其他检测的大豆膜融合基因同源物包括一个含内体溴结构域蛋白1（Bro1）、syntaxin6（SYP6）、SYP131、SYP71、SYP8、Bet1、外被体ε（ε - COP）、外被体ζ（ζ - COP）旁系同源物以及一种内质网到高尔基体组分（ERGIC）蛋白。此外，还研究了在调控子背景下发挥作用的生化途径的有效性，包括木葡聚糖木糖基转移酶（XXT）、网状番荔枝碱氧化酶（RO）和棉子糖合酶（GS）。这些实验揭示了调控子在根部防御过程中的重要性，并展示了胼胝质的沉积与该过程的关系。
