Junior Research Group, Cluster of Excellence CellNetworks, Heidelberg, Germany.
Heidelberg University Biochemistry Center, Heidelberg, Germany.
Life Sci Alliance. 2020 Jul 14;3(9). doi: 10.26508/lsa.202000714. Print 2020 Sep.
Coat protein complex I (COPI)-coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.
衣被蛋白复合物 I（COPI）-被膜小泡介导高尔基体潴腔之间的膜运输以及从高尔基体向内质网回收蛋白质。该蛋白复合物衣被由几个 COPI 衣被的类似物定义。然而，COPI 蛋白类似物是否具有特定的功能目前尚不清楚。在这里，我们表明，在小鼠多能细胞的神经元分化过程中，类似物衣被蛋白复合物的亚基 γ1-COP 和 γ2-COP 表达水平不同。此外，通过基因组编辑实验的组合，我们证明尽管 γ-COP 类似物在功能上基本是冗余的，但 γ1-COP 特异性地促进神经突生长。我们的工作强调了 COPI 途径在神经元极化中的作用，并为衣被蛋白复合物类似物亚基在该过程中的不同功能提供了证据。
