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变形链球菌磷酸转移酶系统的乳糖特异性酶II（lacE）基因和磷酸β-半乳糖苷酶（lacG）基因的分离、特性鉴定及核苷酸序列分析

Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene.

作者信息

Honeyman A L, Curtiss R

机构信息

Department of Biology, Washington University, St Louis, MO 63130.

出版信息

J Gen Microbiol. 1993 Nov;139(11):2685-94. doi: 10.1099/00221287-139-11-2685.

DOI:10.1099/00221287-139-11-2685
PMID:8277252
Abstract

The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. The S. mutans lacE and lacG genes are located in the same operon as the tagatose genes. S. mutans metabolizes lactose via the tagatose phosphate pathway. The deduced LacE and LacG proteins of S. mutans display high homology with the corresponding proteins from Lactococcus lactis, Staphylococcus aureus and Lactobacillus casei.

摘要

变形链球菌的lacE和lacG基因已被分离和鉴定，并测定了它们的核苷酸序列。lacE基因编码磷酸烯醇丙酮酸依赖性磷酸转移酶系统（PTS）中乳糖特异性的酶II组分。lacG基因编码磷酸β-半乳糖苷酶，该酶可切割由乳糖PTS形成的乳糖磷酸酯。变形链球菌的lacE和lacG基因与塔格糖基因位于同一个操纵子中。变形链球菌通过磷酸塔格糖途径代谢乳糖。推导的变形链球菌LacE和LacG蛋白与乳酸乳球菌、金黄色葡萄球菌和干酪乳杆菌的相应蛋白具有高度同源性。

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