Brouland J P, Verdier F, Patriarca C, Vial T, Descotes J
Laboratoire d'Immunotoxicologie Fondamentale et Clinique, INSERM U80, Faculté de Médecine A. Carrel, Lyon, France.
J Toxicol Environ Health. 1994 Jan;41(1):95-108. doi: 10.1080/15287399409531829.
The popliteal lymph node (PLN) assay has been proposed as a tool to predict in rodents those xenobiotics likely to induce autoimmune reactions in humans. To further validate this assay and to study the mechanisms involved, histologic changes in PLNs from rats injected with streptozotocin, diphenylhydantoin, pure acetone, or 50% ethanol were compared to a local graft-versus-host (GvH) reaction. This study suggests that routine histology of PLNs is instrumental to discard primary irritants. In addition, the hypothesis of a GvH-like mechanism in positive PLN responses is supported by the finding that the reference compounds streptozotocin and diphenylhydantoin produced histologic changes similar to a "true" local GvH reaction.
腘淋巴结（PLN）试验已被提议作为一种工具，用于在啮齿动物中预测那些可能在人类中诱发自身免疫反应的异源生物。为了进一步验证该试验并研究其中涉及的机制，将注射链脲佐菌素、苯妥英、纯丙酮或50%乙醇的大鼠的PLN组织学变化与局部移植物抗宿主（GvH）反应进行了比较。这项研究表明，PLN的常规组织学有助于排除原发性刺激物。此外，阳性PLN反应中存在类似GvH机制的假设得到了以下发现的支持，即参考化合物链脲佐菌素和苯妥英产生的组织学变化类似于“真正的”局部GvH反应。
