类病毒诱导的番茄病程相关蛋白P23的cDNA克隆。鉴定为一种液泡抗真菌因子。
cDNA cloning of viroid-induced tomato pathogenesis-related protein P23. Characterization as a vacuolar antifungal factor.
作者信息
Rodrigo I, Vera P, Tornero P, Hernández-Yago J, Conejero V
机构信息
Departamento de Biotecnología, Universidad Politécnica de Valencia, Spain.
出版信息
Plant Physiol. 1993 Jul;102(3):939-45. doi: 10.1104/pp.102.3.939.
Abstract
A 23-kD pathogenesis-related protein (P23) is induced in tomato (Lycopersicon esculentum Mill, cv Rutgers) plants when infected with citrus exocortis viroid. This protein is homologous to the salt-induced tomato NP24 protein (I. Rodrigo, P. Vera, R. Frank, V. Conejero [1991] Plant Mol Biol 16: 931-934). Further characterization of P23 has shown that this protein accumulates in vacuoles in association with dense inclusion bodies. In vitro assays indicated that the purified P23 protein inhibits the growth of several phytopathogenic fungi. P23-coding cDNA clones were isolated from viroid-induced and ethylene-induced libraries. Southern analysis showed that at least two genes could encode P23 or P23-related products. The accumulation of P23 protein correlated with the accumulation of its mRNA. Sequence analysis revealed significant differences in both coding and downstream untranslated regions between the cDNA sequences corresponding to the viroid-induced P23 and the salt stress-induced NP24 proteins.
摘要
一种23-kD病程相关蛋白（P23）在感染柑橘裂皮类病毒的番茄（Lycopersicon esculentum Mill，cv Rutgers）植株中被诱导产生。该蛋白与盐诱导的番茄NP24蛋白同源（I. Rodrigo、P. Vera、R. Frank、V. Conejero [1991] Plant Mol Biol 16: 931-934）。对P23的进一步表征表明，该蛋白与致密包涵体相关联地积累在液泡中。体外试验表明，纯化的P23蛋白抑制几种植物病原真菌的生长。从类病毒诱导和乙烯诱导的文库中分离出P23编码的cDNA克隆。Southern分析表明，至少有两个基因可以编码P23或P23相关产物。P23蛋白的积累与其mRNA的积累相关。序列分析显示，与类病毒诱导的P23和盐胁迫诱导的NP24蛋白相对应的cDNA序列在编码区和下游非翻译区均存在显著差异。
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