Terpilovskaia O N, Tret'iak T M, Zelepuga E A, Ponomareva R B
Vopr Med Khim. 1993 Sep-Oct;39(5):41-3.
Penetration of exogenous RNAases, native and albumin polymer-bound, from circulation into the brain parenchyma was studied. Native pancreatic RNAase was found to extend through the hematoencephalic barrier; maximal enzymatic activity was detected within 15-30 min after administration into a rat tail vein. The enzyme activity was not observed within 90 min both in blood serum and brain tissue. The conjugate of RNAase and human albumin with molecular mass of 300,000 penetrated through the hematoencephalic barrier. Maximal enzymatic activity was estimated within 3-5 hrs after administration and maintained rather high within 24 hrs in blood and brain parenchyma. This suggests that the preparations of this kind are promising in experimental medicine.
研究了天然的以及与白蛋白聚合物结合的外源性核糖核酸酶从循环系统进入脑实质的情况。发现天然胰腺核糖核酸酶可穿过血脑屏障；将其注入大鼠尾静脉后15 - 30分钟内检测到最大酶活性。在血清和脑组织中90分钟内均未观察到酶活性。分子量为300,000的核糖核酸酶与人白蛋白的缀合物穿过了血脑屏障。给药后3 - 5小时内估计达到最大酶活性，并且在血液和脑实质中24小时内都保持相当高的水平。这表明这类制剂在实验医学中具有前景。
