Rabinovich S E, Platonova L V, Diuzheva T G
Vopr Med Khim. 1993 Sep-Oct;39(5):58-61.
When Hb AIc was estimated by affinity chromatography where boron phenyl-agarose was used as a sorbent, the highest rate of Hb AIc binding with the sorbent was detected at pH 8.5-9.0 while the maximal elution of the protein occurred at 0.3-0.5 M concentration of sorbitol used as an eluent. Content of Hb AIc, estimated in 56 patients with insulin-dependent diabetes mellitus, constituted 12.3 +/- 0.4%, while in 20 healthy volunteers it was equal to 5.4 +/- 0.2%, which are consistent with the literature data obtained by other methods. At the same time, the data of Hb AIc estimation by means of affinity chromatography correlated exactly with the results of ion exchange chromatography (r = 0.98), thus corroborating the validity of the procedure used. Only the stable ketoamine fraction of Hb AIc was found to interact with boron phenyl-agarose.
当使用硼苯基琼脂糖作为吸附剂通过亲和色谱法估算糖化血红蛋白（Hb AIc）时，在pH 8.5 - 9.0条件下检测到Hb AIc与吸附剂的结合率最高，而当使用山梨醇作为洗脱剂，浓度为0.3 - 0.5 M时，蛋白质的洗脱量最大。在56例胰岛素依赖型糖尿病患者中估算的Hb AIc含量为12.3±0.4%，而在20名健康志愿者中该含量为5.4±0.2%，这与通过其他方法获得的文献数据一致。同时，通过亲和色谱法估算Hb AIc的数据与离子交换色谱法的结果精确相关（r = 0.98），从而证实了所采用方法的有效性。仅发现Hb AIc的稳定酮胺部分与硼苯基琼脂糖相互作用。
