Talwar D, Barr B B, Kesson C M, Robb D A
Clin Chim Acta. 1983 Feb 28;128(1):61-7. doi: 10.1016/0009-8981(83)90055-4.
Estimation of adult glycosylated haemoglobin by affinity chromatography was found to be quick and less dependent on ionic strength, pH and temperature than ion-exchange chromatography. Results obtained by both procedures correlated strongly (r = 0.96) but the range for normal subjects was smaller with the affinity assay. The affinity method correlated equally well with the colorimetric assay (r = 0.95). However, the method did not measure all the glycosylated forms, and only half of the glycosylated species isolated by ion-exchange chromatography was bound to the affinity resin. It also showed that the amount of glycosylated haemoglobin in cord blood is less than in adult blood.
通过亲和色谱法估计成人糖化血红蛋白，结果发现该方法快速，且与离子交换色谱法相比，受离子强度、pH值和温度的影响较小。两种方法所得结果相关性很强（r = 0.96），但亲和测定法得出的正常受试者范围较小。亲和法与比色测定法的相关性同样良好（r = 0.95）。然而，该方法并未检测到所有糖化形式，离子交换色谱法分离出的糖化物质只有一半与亲和树脂结合。研究还表明，脐血中糖化血红蛋白的含量低于成人血液中的含量。
