Qian Z L, Deelder A M
Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.
Am J Trop Med Hyg. 1993 Dec;49(6):777-82. doi: 10.4269/ajtmh.1993.49.777.
Based on the fact that schistosomiasis patients in both the acute and chronic phase of the infection show a strong humoral immune response against the gut-associated circulating cathodic antigen, a simple and sensitive dot immunobinding assay for schistosomiasis japonica was developed. Circulating cathodic antigen that had been purified by immunoadsorption using monoclonal antibody was biotinylated with biotin aminocaproylhydrazide via the carbohydrate moiety of the antigen. Serum samples dotted onto nitrocellulose strips were then tested in an assay involving a combined incubation step of biotinylated antigen and streptavidin peroxidase, and a subsequent staining; the total assay time was 1.5 hr. Assaying the sera of 105 uninfected controls and 104 Schistosoma japonicum-infected individuals showed a specificity of 99.0%, and sensitivities of 96.2% (acute infections) and 94.1% (chronic infections). The described assay is economic, rapid, and reproducible and lends itself to use under field conditions.
基于日本血吸虫病急性和慢性感染期患者均对肠道相关循环阴极抗原表现出强烈体液免疫反应这一事实，开发了一种简单且灵敏的日本血吸虫病斑点免疫结合检测法。使用单克隆抗体通过免疫吸附纯化的循环阴极抗原，经生物素氨基己酰肼通过抗原的碳水化合物部分进行生物素化。点样在硝酸纤维素条上的血清样本随后在一个检测中进行检测，该检测包括生物素化抗原与链霉亲和素过氧化物酶的联合孵育步骤以及随后的染色；整个检测时间为1.5小时。对105名未感染对照和104名日本血吸虫感染个体的血清进行检测，结果显示特异性为99.0%，敏感性在急性感染中为96.2%，在慢性感染中为94.1%。所描述的检测方法经济、快速且可重复，适用于现场条件下的使用。
