基于单克隆抗体的夹心酶联免疫吸附测定法检测中国患者血清中的日本血吸虫抗原（Sj31/32）
Detection of Schistosoma japonicum antigen (Sj31/32) in sera of Chinese patients using a sandwich ELISA based on monoclonal antibody.
作者信息
Li Y L, Song W J, Han J J, Ruppel A
机构信息
Department of Parasitology, Tongji Medical University, Wuhan, P.R. China.
出版信息
Trop Med Parasitol. 1994 Jun;45(2):115-8.
A sandwich-ELISA was developed using IgG from rabbits infected with Schistosoma japonicum as capture antibody and a monoclonal antibody as detecting reagent for circulating Sj 31/32 in patient sera. The assay detected circulating antigen in 36 of 53 sera from Chinese schistosomiasis japonica patients, but not in sera from healthy persons or patients with paragonimiasis, trichinosis or cysticercosis. This is the first demonstration that Sj 31/32 proteins may be used for circulating antigen-based serodiagnosis of schistosomiasis.
利用感染日本血吸虫的兔的IgG作为捕获抗体，单克隆抗体作为检测试剂，建立了夹心酶联免疫吸附测定法（sandwich-ELISA），用于检测患者血清中循环的日本血吸虫31/32（Sj 31/32）抗原。该检测法在53例中国日本血吸虫病患者的血清中检测到36例循环抗原，但在健康人或肺吸虫病、旋毛虫病或囊尾蚴病患者的血清中未检测到。这首次证明Sj 31/32蛋白可用于基于循环抗原的血吸虫病血清学诊断。
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