Wolk C P, Shaffer P W
Arch Microbiol. 1976 Nov 2;110(23):145-7. doi: 10.1007/BF00690221.
Anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of Allen and Arnon solidified with 0.5% agarose and supplemented with 5 mM fructose. The organism can be grown exponentially (tau2 approximately or equal to 36 h) in fermentor cultures in the dark, fixing N2, to a density of greater than 10 g dry weight/l.
多变鱼腥藻可以在黑暗中从含有一个或几个细胞的片段进行克隆，在以0.5%琼脂糖固化并添加5 mM果糖的艾伦和阿农无氮培养基上，克隆效率约为40%。该生物体可以在黑暗中的发酵罐培养物中进行指数生长（代时约为36小时），固定氮气，密度可达大于10克干重/升。
