van Baalen C
Univcrsity of Texas Marine Science Institute, Port Aransas, Texas 78373.
J Phycol. 1967 Sep;3(3):154-7. doi: 10.1111/j.1529-8817.1967.tb04650.x.
Initiation of growth (lag) and subsequent growth to visible colonies was examined for single cells of several coccoid blue-nreen algae under controlled incuba-tion conditions. At 30 or 39 C, with tungsten or fluorescent illumination, organisms such as Agmenel-lum quadruplicatum, strains PR-6 and strain BG-J, showed no evidence of lag associated with initiation of growth. The final colony count was within 20% of the expected number derived from cell counts and serial dilutions. For Anacystis nidulnns, Tx 20, a new medium C, 10 with EDTA as chelator was developed. In this medium groiuth of single cells at 39 C was excellent with quantitative recovery and no evidence of lag. At 30 C, however, Tx 20 showed anomalous behavior, lag and nonquantitative cell recovery. This behavior at 30 C is not yet understood.
在可控的培养条件下，对几种球状蓝绿藻的单细胞进行了生长起始（滞后期）及随后生长至可见菌落的研究。在30℃或39℃，使用钨丝灯或荧光照明时，诸如四倍体阿氏颤藻、PR - 6菌株和BG - J菌株等生物，未显示出与生长起始相关的滞后期迹象。最终菌落计数在通过细胞计数和系列稀释得出的预期数量的20%以内。对于集胞藻属的集胞藻Tx 20，开发了一种以EDTA作为螯合剂的新培养基C10。在这种培养基中，单细胞在39℃时生长良好，数量可定量恢复且无滞后期迹象。然而，在30℃时，Tx 20表现出异常行为，有滞后期且细胞恢复数量不定。其在30℃时的这种行为尚不清楚。
