Danchin A, Pidoux J, Krin E, Thompson C J, Ullmann A
Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1993 Dec 1;114(2):145-51. doi: 10.1111/j.1574-6968.1993.tb06565.x.
A DNA fragment of Streptomyces coelicolor encoding the carboxy-terminal catalytic domain of adenylate cyclase was cloned, sequenced and expressed in an Escherichia coli cya-defective strain where it produced nanomole levels of cAMP. The amino acid sequence of the enzyme displays similarities with the Brevibacterium liquefaciens pyruvate regulated adenylate cyclase.
克隆了天蓝色链霉菌的一段DNA片段,该片段编码腺苷酸环化酶的羧基末端催化结构域,进行了测序,并在大肠杆菌cya缺陷型菌株中表达,在该菌株中它产生了纳摩尔水平的环磷酸腺苷(cAMP)。该酶的氨基酸序列与液化短杆菌丙酮酸调节的腺苷酸环化酶具有相似性。