Suzuki K, Miyata M, Fukumura T
Department of Biology, Faculty of Science, Osaka City University, Japan.
FEMS Microbiol Lett. 1993 Dec 1;114(2):229-33. doi: 10.1111/j.1574-6968.1993.tb06578.x.
Polymerase chain reaction was carried out to amplify the conserved region (789 bp in the case of Mycoplasma capricolum) of the dnaA gene (1350 bp in the case of M. capricolum) of 15 representatives of the class Mollicutes using degenerate oligonucleotide primers. The dnaA gene fragments were amplified from M. mycoides subsp. capri, Spiroplasma apis and S. citri. The amino acid sequences deduced from the nucleotide sequences of the amplified fragments showed very low similarities to those of the corresponding regions of four walled bacteria. The values of similarity between any two of the three mollicute species were lower than those between any two of the four walled bacteria.
使用简并寡核苷酸引物对柔膜菌纲15个代表菌株的dnaA基因（山羊支原体中为1350 bp）的保守区域（山羊支原体中为789 bp）进行聚合酶链反应扩增。从丝状支原体山羊亚种、蜜蜂螺旋体和柑橘螺旋体中扩增出了dnaA基因片段。从扩增片段的核苷酸序列推导的氨基酸序列与革兰氏阳性菌相应区域的氨基酸序列相似度非常低。这三种柔膜菌纲物种中任意两者之间的相似度值低于革兰氏阳性菌中任意两者之间的相似度值。
