Hilbert H, Himmelreich R, Plagens H, Herrmann R
Zentrum für Molekulare Biologie Heidelberg, Universität Heidelberg, Germany.
Nucleic Acids Res. 1996 Feb 15;24(4):628-39. doi: 10.1093/nar/24.4.628.
To sequence the entire 800 kilobase pair genome of the bacterium Mycoplasma pneumoniae, a plasmid library was established with contained the majority of the EcoR1 fragments from M.pneumoniae. The EcoR1 fragments were subcloned from an ordered cosmid library comprising the complete M.pneumoniae genome. Individual plasmid clones were sequenced in an ordered fashion mainly by primer walking. We report here the initial results from the sequence analysis of -56 kb comprising the dnaA region as a potential origin of replication, the ATPase operon and a region coding for a cluster of ribosomal protein genes. The data were compared with the corresponding genes/operons from Bacillus subtilis, Escherichia coli, Mycoplasma capricolum and Mycoplasma gallisepticum.
为了对肺炎支原体细菌的整个800千碱基对基因组进行测序，构建了一个质粒文库，其中包含来自肺炎支原体的大部分EcoR1片段。EcoR1片段是从包含完整肺炎支原体基因组的有序黏粒文库中进行亚克隆的。主要通过引物步移法按顺序对各个质粒克隆进行测序。我们在此报告对约56 kb的序列分析初步结果，该区域包含作为潜在复制起点的dnaA区域、ATPase操纵子以及一个编码核糖体蛋白基因簇的区域。将这些数据与枯草芽孢杆菌、大肠杆菌、山羊支原体和鸡毒支原体的相应基因/操纵子进行了比较。
