Lam K S, Lebl M, Krchnak V, Wade S, Abdul-Latif F, Ferguson R, Cuzzocrea C, Wertman K
Arizona Cancer Center, Tucson.
Gene. 1993 Dec 27;137(1):13-6. doi: 10.1016/0378-1119(93)90245-x.
When all L-amino acid (aa) random peptide libraries synthesized on solid-phase particles were screened (Selectide Technology), we identified several peptide ligands (YG_F_) that interacted specifically with an anti-beta-endorphin monoclonal antibody (clone 3E7) (single-letter aa symbols; symbols '_' indicate variable aa). Here, we report on the screening of three different D-aa-containing pentapeptide libraries (XxXxX, xXxXx and xxxxx, wherein X = L-aa, and x = D-aa) with the same antibody, in which several D-aa-containing ligands were identified. The binding affinities of many of these D-aa-containing ligands were as least two orders of magnitude lower than that of YGGFL, for which the Ki is 17.5 nM.
当对在固相颗粒上合成的所有L-氨基酸（aa）随机肽文库进行筛选（Selectide技术）时，我们鉴定出了几种与抗β-内啡肽单克隆抗体（克隆3E7）特异性相互作用的肽配体（YG_F_）（单字母氨基酸符号；符号“_”表示可变氨基酸）。在此，我们报告了用同一抗体对三个不同的含D-氨基酸五肽文库（XxXxX、xXxXx和xxxxx，其中X = L-氨基酸，x = D-氨基酸）进行的筛选，从中鉴定出了几种含D-氨基酸的配体。这些含D-氨基酸配体中的许多的结合亲和力比YGGFL（其Ki为17.5 nM）至少低两个数量级。
